3J4B
Structure of T7 gatekeeper protein (gp11)
EM specimen
| Vitrification | Yes |
| Instrument | LEICA EM CPC |
| Method | Samples were applied to grids for 1 minute, blotted and plunged into liquid ethane. |
EM Data Collection
| Microscope | FEI TECNAI F20 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.26 |
| Detector | FEI EAGLE (4k x 4k) |
| Number of Images | 264 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 12 |
| Software | UCSF Chimera,EMAN,SPIDER,Xmipp |
| Symmetry | C12 |
