3J4B
Structure of T7 gatekeeper protein (gp11)
EM specimen
Vitrification | Yes |
Instrument | LEICA EM CPC |
Method | Samples were applied to grids for 1 minute, blotted and plunged into liquid ethane. |
EM Data Collection
Microscope | FEI TECNAI F20 |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 200 |
Cs (mm) | 2.26 |
Detector | FEI EAGLE (4k x 4k) |
Number of Images | 264 |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 12 |
Software | UCSF Chimera,EMAN,SPIDER,Xmipp |
Symmetry | C12 |