3J48
Cryo-EM structure of Poliovirus 135S particles
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
| Method | Blotted manually before plunge freezing into liquid ethane |
EM Data Collection
| Microscope | FEI TECNAI F20 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2 |
| Detector | TVIPS TEMCAM-F415 (4k x 4k) |
| Number of Images | 1020 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 5.5 |
| Software | Coot,REFMAC,FREALIGN |
| Symmetry | I |
