3J47
Formation of an intricate helical bundle dictates the assembly of the 26S proteasome lid
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Detector | TVIPS TEMCAM-F816 (8k x 8k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 7.4 |
| Software | EMTEGRATOR,iMODFIT,VOLTRAC |
| Symmetry | C1 |
