3J45
Structure of a non-translocating SecY protein channel with the 70S ribosome
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
EM Data Collection
| Microscope | FEI TECNAI F20 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2 |
| Detector | KODAK SO-163 FILM |
| Number of Images | 360 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 9.5 |
| Software | MDFF,UCSF Chimera,EMAN |
| Symmetry | C1 |
