3J40
Validated Near-Atomic Resolution Structure of Bacteriophage Epsilon15 Derived from Cryo-EM and Modeling
Replaces: 3C5BEM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK II |
| Method | Blot before plunging. |
EM Data Collection
| Microscope | JEOL 3200FSC |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 4.1 |
| Energy Filter | in-column filter |
| Detector | KODAK SO-163 FILM |
| Number of Images | 1309 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.5 |
| Software | EMAN,jspr |
| Symmetry | I |
