3J3W
Atomic model of the immature 50S subunit from Bacillus subtilis (state II-a)
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
| Method | Blot for 20 seconds before plunging |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | FEI EAGLE (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 10.7 |
| Software | MDFF,MODELLER,modeRNA,S2S,RELION |
| Symmetry | C1 |
