3J3P
Conformational Shift of a Major Poliovirus Antigen Confirmed by Immuno-Cryogenic Electron Microscopy: 135S Poliovirus and C3-Fab Complex
EM specimen
| Vitrification | Yes |
| Method | Blotted manually before plunging |
EM Data Collection
| Microscope | FEI/PHILIPS CM200FEG |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 120 |
| Cs (mm) | 2 |
| Detector | KODAK SO-163 FILM |
| Number of Images | 4 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 9.1 |
| Software | CHARMM,EM3DR |
| Symmetry | I |
