3J34
Structure of HIV-1 Capsid Protein by Cryo-EM
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
| Method | With 2.5 uL sample on carbon side, add 3 uL dilution buffer (100 mM NaCl, 50 mM Tris, pH 8.0) to back side. Blot 3-5 seconds from back side. |
EM Data Collection
| Microscope | FEI POLARA 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2 |
| Detector | KODAK SO-163 FILM |
| Number of Images | 27 |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 8.6 |
| Software | MDFF,FREALIGN |
