3J32
An asymmetric unit map from electron cryo-microscopy of Haliotis diversicolor molluscan hemocyanin isoform 1 (HdH1)
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
| Method | blot for 2 seconds before plunging |
EM Data Collection
| Microscope | JEOL 3200FSC |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 4.1 |
| Energy Filter | In-column Omega Filter |
| Detector | KODAK SO-163 FILM |
| Number of Images | 784 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.5 |
| Software | EMAN |
| Symmetry | D5 |
