3J2X
Electron Cryo-microscopy of Chikungunya VLP in complex with neutralizing antibody Fab m242
EM specimen
| Vitrification | Yes |
| Instrument | GATAN CRYOPLUNGE 3 |
| Method | Blot for 2 seconds before plunging |
EM Data Collection
| Microscope | FEI/PHILIPS CM300FEG/HE |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2 |
| Number of Images | 62 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 15.6 |
| Software | EMfit,EMAN |
| Symmetry | I |
