EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
| Method | Blot for 2 seconds before plunging |
EM Data Collection
| Microscope | FEI TECNAI F20 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 120 |
| Cs (mm) | 2 |
| Detector | KODAK SO-163 FILM |
| Number of Images | 400 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 9.5 |
| Software | Rosetta,Situs,UCSF Chimera,EMAN |
| Symmetry | C7 |
