3J2S
Membrane-bound factor VIII light chain
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
| Method | Blot for 3.5 seconds before plunging |
EM Data Collection
| Microscope | JEOL 2010F |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2 |
| Detector | GATAN ULTRASCAN 4000 (4k x 4k) |
| Number of Images | 69 |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 15 |
| Software | UCSF Chimera,EMAN,IHRSR,SPIDER |
