3J2B
Dissecting the in vivo assembly of the 30S ribosomal subunit reveals the role of RimM
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
| Method | Blot for 1 seconds before plunging |
EM Data Collection
| Microscope | FEI TECNAI F20 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Detector | GATAN ULTRASCAN 4000 (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 13.6 |
| Software | MDFF,SPIDER |
| Symmetry | C1 |
