3J25
Structural basis for TetM-mediated tetracycline resistance
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Detector | TVIPS TEMCAM-F416 (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 7.2 |
| Software | Coot,DireX,UCSF Chimera,SPIDER |
| Symmetry | C1 |
