3J24
CryoEM reconstruction of complement decay-accelerating factor
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
EM Data Collection
| Microscope | FEI/PHILIPS CM300FEG/T |
| Electron Gun | TUNGSTEN HAIRPIN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2 |
| Detector | KODAK SO-163 FILM |
| Number of Images | 36 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 9 |
| Software | CTFFIND,UCSF Chimera,autopp,RobEM,Auto3DEM |
| Symmetry | I |
