3J1Z
Inward-Facing Conformation of the Zinc Transporter YiiP revealed by Cryo-electron Microscopy
EM specimen
| Vitrification | Yes |
| Instrument | GATAN CRYOPLUNGE 3 |
| Method | Blot for 2-5 seconds before plunging |
EM Data Collection
| Microscope | FEI TECNAI F20 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.1 |
| Detector | GENERIC FILM |
| Number of Images | 19 |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 13 |
| Software | EMIP,SPARX |
