3J15

Model of ribosome-bound archaeal Pelota and ABCE1

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK I
Method	Blot for 10 seconds before plunging, use 2 layer of filter paper

EM Data Collection

Microscope	FEI TITAN KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	200
Detector	TVIPS TEMCAM-F416 (4k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	6.6
Software	SPIDER
Symmetry	C1