3J0G
Homology model of E3 protein of Venezuelan Equine Encephalitis Virus TC-83 strain fitted with a cryo-EM map
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK I |
| Method | 2 blots, each blot 2 second before plunging, offset = 0 |
EM Data Collection
| Microscope | JEOL 3200FSC |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 4.3 |
| Energy Filter | In-column Omega Filter |
| Detector | GATAN ULTRASCAN 4000 (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.8 |
| Software | UCSF Chimera,EMAN |
| Symmetry | I |
