3J07
Model of a 24mer alphaB-crystallin multimer
Spectrometer
Experimental method: ELECTRON MICROSCOPY, SOLID-STATE NMR, SOLUTION SCATTERING
| Spectrometer ID | Spectrometer maker | Spectrometer model | Spectrometer type | Spectrometer field strength |
| 1 | Bruker | AVANCE | Bruker Avance | 400 |
| 2 | Bruker | AVANCE | Bruker Avance | 600 |
| 3 | Bruker | AVANCE | Bruker Avance | 700 |
| 4 | Bruker | AVANCE | Bruker Avance | 900 |
Experiment
| experiment id | conditions id | solution id | Experiment type |
| 1 | 1 | 1 | 2D 13C-13C PDSD |
| 10 | 1 | 5 | 2D 15N-13C TEDOR |
| 11 | 1 | 4 | 2D 15N-13C NHHC |
| 12 | 1 | 5 | 2D 15N-13C NHHC |
| 13 | 1 | 4 | 2D 15N-13C PAIN |
| 14 | 1 | 3 | 2D 1H-13C INEPT |
| 2 | 1 | 2 | 2D 13C-13C PDSD |
| 3 | 1 | 3 | 2D 13C-13C CHHC |
| 4 | 1 | 3 | 2D 13C-13C PDSD |
| 5 | 1 | 1 | 3D 15N-13C-13C NCACX |
| 6 | 1 | 2 | 3D 15N-13C-13C NCACX |
| 7 | 1 | 1 | 3D 15N-13C-13C NCOCX |
| 8 | 1 | 2 | 3D 15N-13C-13C NCOCX |
| 9 | 1 | 4 | 2D 15N-13C TEDOR |
NMR Sample
| conditions id | NMR sample pH | NMR sample pressure | NMR sample temperature |
| 1 | 7.6 | 270 |
Conformers
| Conformers Calculated Total Number | 200 |
| Conformers Submitted Total Number | 1 |
Refinement Statistics
Experimental method: ELECTRON MICROSCOPY, SOLID-STATE NMR, SOLUTION SCATTERING
| Cell axes | 1.000 | 1.000 | 1.000 |
| Cell angles | 90.00 | 90.00 | 90.00 |
| Spacegroup | P 1 | ||
| Resolution limits | - | ||
EM specimen
| Staining | Yes |
| Staining material | Uranyl Acetate |
EM Data Collection
| Microscope | JEOL 100CX |
| Electron Gun | OTHER |
| Accelerating Voltage (kV) | 100 |
| Detector | KODAK SO-163 FILM |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 20 |
| Symmetry | T |
