3J06
CryoEM Helical Reconstruction of TMV
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
| Method | 10 second wait time, 1-2 second blot time, 1-2 second drain time, blot force 1 |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | KODAK SO-163 FILM |
| Number of Images | 386 |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 3.3 |
| Software | EMAN,IHRSR |
