3J04
EM structure of the heavy meromyosin subfragment of Chick smooth muscle Myosin with regulatory light chain in phosphorylated state
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
| Method | Blot for 4 sec before plunging |
EM Data Collection
| Microscope | FEI/PHILIPS CM300FEG/T |
| Electron Gun | TUNGSTEN HAIRPIN |
| Accelerating Voltage (kV) | 300 |
| Detector | KODAK SO-163 FILM |
| Number of Images | 85 |
3D Reconstruction
| Method | CRYSTALLOGRAPHY |
| Resolution (Å) | 20 |
| Software | CCP4,NMFF |
