3IYQ
tmRNA-SmpB: a journey to the center of the bacterial ribosome
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK I |
| Method | Blot for 5 seconds before plunging |
EM Data Collection
| Microscope | JEOL 2200FS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2 |
| Energy Filter | In-column Omega Filter |
| Detector | KODAK SO-163 FILM |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 13 |
| Software | MDFF,UCSF Chimera,IMAGIC |
| Symmetry | C1 |
