3IYL

Atomic CryoEM Structure of a Nonenveloped Virus Suggests How Membrane Penetration Protein is Primed for Cell Entry

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK I
Method	Blot for 7-9 seconds before plunging

EM Data Collection

Microscope	FEI TITAN KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Cs (mm)	2.7
Detector	KODAK SO-163 FILM

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	3.3
Software	FREALIGN,IMIRS
Symmetry	I