3IYH
P22 procapsid coat protein structures reveal a novel mechanism for capsid maturation: Stability without auxiliary proteins or chemical cross-links
EM specimen
Vitrification | Yes |
Instrument | HOMEMADE PLUNGER |
Method | blot 2-3 seconds before plunging |
EM Data Collection
Microscope | FEI POLARA 300 |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 200 |
Cs (mm) | 2.3 |
Detector | KODAK SO-163 FILM |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 8.2 |
Software | Coot,MODELLER,Auto3DEM |
Symmetry | I |