3IYG
Ca model of bovine TRiC/CCT derived from a 4.0 Angstrom cryo-EM map
EM specimen
| Vitrification | Yes |
| Method | two-side blotting for 1 second before plunging |
EM Data Collection
| Microscope | JEOL 3200FSC |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 4.1 |
| Energy Filter | In-column Omega Filter |
| Detector | KODAK SO-163 FILM |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4 |
| Software | Coot,MODELLER,UCSF Chimera,EMAN |
| Symmetry | C2 |
