3IXV
Scorpion Hemocyanin resting state pseudo atomic model built based on cryo-EM density map
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK I |
| Method | two side bloting for 1 seconds before plunging |
EM Data Collection
| Microscope | JEOL 3200FSC |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 4.1 |
| Energy Filter | In-column Omega Filter |
| Detector | KODAK SO-163 FILM |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 6.8 |
| Software | MODELLER,Situs,UCSF Chimera,EMAN |
| Symmetry | C2 |
