3IXC
Crystal structure of hexapeptide transferase family protein from Anaplasma phagocytophilum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-25 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 92.091, 92.091, 92.091 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.610 |
| R-factor | 0.176 |
| Rwork | 0.176 |
| R-free | 0.19400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xhd |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.201 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.670 |
| High resolution limit [Å] | 1.610 | 1.610 |
| Rmerge | 0.086 | 0.395 |
| Number of reflections | 32905 | |
| <I/σ(I)> | 12.46 | 2.09 |
| Completeness [%] | 96.9 | 84.2 |
| Redundancy | 3.2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | Hampton CSHT condition D12, 2.0 M ammonium phosphate, 0.1 M Tris pH 8.5, 26.5 mg/mL protein, crystal tracking ID 204803d12, expression tag not removed prior to crystallization, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
