3IXA
Human Class I MHC HLA-A2(A150P) in complex with the Tax peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-21 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.891, 85.295, 82.484 |
| Unit cell angles | 90.00, 89.84, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.188 |
| Rwork | 0.186 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tvb |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.789 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.064 | 0.431 |
| Number of reflections | 48760 | |
| <I/σ(I)> | 9.1 | 1.73 |
| Completeness [%] | 97.1 | 76.3 |
| Redundancy | 3.5 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 277 | PEG3350 24%, MES 0.025M, NaF 0.1M, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
