3IWZ
The c-di-GMP Responsive Global Regulator CLP Links Cell-Cell Signaling to Virulence Gene Expression in Xanthomonas campestris
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97966 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.690, 67.690, 110.370 |
| Unit cell angles | 90.00, 104.20, 90.00 |
Refinement procedure
| Resolution | 29.850 - 2.300 |
| R-factor | 0.244 |
| Rwork | 0.244 |
| R-free | 0.30500 |
| Structure solution method | MIR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.280 |
| Rmerge | 0.039 | 0.400 |
| Number of reflections | 42637 | |
| <I/σ(I)> | 24.5 | 3.1 |
| Completeness [%] | 97.1 | 94 |
| Redundancy | 4.3 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | Tris 8.5, PEG 3350 20%, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
