3IWR
Crystal structure of class I chitinase from Oryza sativa L. japonica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-20 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 65 |
| Unit cell lengths | 117.082, 117.082, 77.971 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 101.530 - 2.570 |
| R-factor | 0.18161 |
| Rwork | 0.179 |
| R-free | 0.23609 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dkv |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.398 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 101.530 | 2.640 |
| High resolution limit [Å] | 2.570 | 2.570 |
| Rmerge | 0.060 | 0.221 |
| Number of reflections | 17963 | |
| <I/σ(I)> | 10.5 | 3.2 |
| Completeness [%] | 97.0 | 79.4 |
| Redundancy | 9.5 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 11%(w/v) PEG 20000, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
