3IWP
Crystal structure of human copper homeostasis protein CutC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 |
Unit cell lengths | 78.160, 78.406, 170.349 |
Unit cell angles | 76.84, 87.88, 71.86 |
Refinement procedure
Resolution | 50.000 - 2.500 |
R-factor | 0.23584 |
Rwork | 0.233 |
R-free | 0.28254 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1x7i |
RMSD bond length | 0.011 |
RMSD bond angle | 1.390 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.060 | 0.299 |
Number of reflections | 127488 | |
<I/σ(I)> | 23.7 | 4.1 |
Completeness [%] | 98.5 | 97.9 |
Redundancy | 3.9 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 0.1 M citric acid, pH4.0, and 10% polyethylene glycol 6000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |