3IVT
Homocitrate Synthase Lys4 bound to 2-OG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-13 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 62 |
| Unit cell lengths | 133.731, 133.731, 125.904 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.470 - 2.670 |
| R-factor | 0.167 |
| Rwork | 0.165 |
| R-free | 0.20600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Homocitrate Synthase Lys4 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.445 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.780 |
| High resolution limit [Å] | 2.670 | 2.670 |
| Rmerge | 0.060 | 0.512 |
| Number of reflections | 36145 | |
| <I/σ(I)> | 16.8 | 4.54 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.5 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 297 | Na/KPO4, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
