3IVF
Crystal structure of the talin head FERM domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 110 |
Detector technology | PIXEL |
Collection date | 2008-12-19 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.725 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 40.260, 72.160, 162.830 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.380 - 1.940 |
R-factor | 0.196 |
Rwork | 0.193 |
R-free | 0.25700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1mix 2kc1 2kc2 |
RMSD bond length | 0.023 |
RMSD bond angle | 1.840 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.1.4) |
Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 72.160 | 2.050 |
High resolution limit [Å] | 1.940 | 1.940 |
Rmerge | 0.109 | 0.387 |
Number of reflections | 34587 | |
<I/σ(I)> | 8.3 | 3.3 |
Completeness [%] | 76.0 | 71 |
Redundancy | 2.9 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 277 | 21% PEG4000, 0.1M Tris, 0.2M ammonium sulphate, pH7.4, VAPOR DIFFUSION, SITTING DROP, temperature 277K |