3IV1
Coiled-coil domain of tumor susceptibility gene 101
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-07 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 56.290, 56.290, 341.850 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.750 - 2.500 |
| Rwork | 0.228 |
| R-free | 0.27650 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.130 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXD |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.650 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 25250 | |
| <I/σ(I)> | 14.87 | 7.78 |
| Completeness [%] | 98.9 | 92.7 |
| Redundancy | 3.77 | 3.66 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 2.4 M AMMONIUM SULFATE, 0.1 M BIS-TRIS, 1 mM DTT, pH 5.50, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
