3IUF

Crystal structure of the C2H2-type zinc finger domain of human ubi-d4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2009-08-06
Detector	ADSC QUANTUM 315
Wavelength(s)	1.26514
Spacegroup name	P 21 21 2
Unit cell lengths	24.714, 57.657, 22.585
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.720 - 1.800
R-factor	0.195
Rwork	0.193
R-free	0.23700
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.491
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	1.830
High resolution limit [Å]	1.800	4.880	1.800
Rmerge	0.118	0.068	0.522
Number of reflections	3263
<I/σ(I)>	7.7
Completeness [%]	99.9	100	98.7
Redundancy	8.2	7.5	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	9.5	291	30% PEG 3000, 0.1M CHES, protein concentration 5mg/mL., pH 9.5, vapor diffusion, temperature 291K
1	VAPOR DIFFUSION	9.5	291	30% PEG 3000, 0.1M CHES, protein concentration 5mg/mL., pH 9.5, vapor diffusion, temperature 291K