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3ITM

Catalytic domain of hPDE2A

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 17-ID
Synchrotron siteAPS
Beamline17-ID
Temperature [K]100
Detector technologyCCD
DetectorADSC QUANTUM 210
Wavelength(s)1.00
Spacegroup nameP 61 2 2
Unit cell lengths108.017, 108.017, 515.563
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution50.000 - 2.490
R-factor0.22982
Rwork0.227
R-free0.28648
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.033
RMSD bond angle2.029
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwareAMoRE
Refinement softwareREFMAC (5.2.0005)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.580
High resolution limit [Å]2.4902.490
Number of reflections54006
<I/σ(I)>19.362.19
Completeness [%]84.338.1
Redundancy6.523.1
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.5293Reservoir - 20% PDEG 3350, 0.2M tri-sodium citrate. Protein - 25mM Hepes pH7.5, 25mM NaCl, 2mM TCEP, 10ug/mL E-64, 1ug/mL pepstatin., VAPOR DIFFUSION, HANGING DROP, temperature 293K

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PDB entries from 2024-11-20

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