3IT7
Crystal Structure of the LasA virulence factor from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-28 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.28402, 1.28348, 1.2574 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.964, 58.838, 146.438 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.570 - 2.140 |
| R-factor | 0.151 |
| Rwork | 0.149 |
| R-free | 0.19500 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.138 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.220 |
| High resolution limit [Å] | 2.140 | 4.610 | 2.140 |
| Rmerge | 0.054 | 0.037 | 0.100 |
| Number of reflections | 32280 | ||
| <I/σ(I)> | 14.7 | ||
| Completeness [%] | 100.0 | 99.9 | 100 |
| Redundancy | 3.7 | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | sodium potassium tartrate 400mM, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
