3IT1
Crystal Structure Francisella tularensis histidine acid phosphatase complexed with L(+)-tartrate
Replaces: 2GLAExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-03-07 |
Detector | NOIR-1 |
Wavelength(s) | 1.12711 |
Spacegroup name | P 41 |
Unit cell lengths | 61.640, 61.640, 211.320 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.688 - 1.691 |
R-factor | 0.185 |
Rwork | 0.184 |
R-free | 0.20700 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.029 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | SOLVE |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.510 | 1.760 |
High resolution limit [Å] | 1.691 | 1.691 |
Rmerge | 0.067 | 0.384 |
Total number of observations | 19664 | |
Number of reflections | 79753 | |
<I/σ(I)> | 12.7 | 2.2 |
Completeness [%] | 91.7 | 84.6 |
Redundancy | 3.29 | 2.66 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 10% (w/v) Tacsimate, 0.1 M HEPES pH 7.0, 19% (w/v) PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K |