3ISV
Crystal structure of glutamate racemase from Listeria monocytogenes in complex with acetate ion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 66.763, 66.763, 258.602 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.850 |
| R-factor | 0.17444 |
| Rwork | 0.172 |
| R-free | 0.22394 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hfr |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.629 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 (MOLREP) |
| Refinement software | REFMAC (5.5.0062) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.084 | 0.621 |
| Number of reflections | 51135 | |
| <I/σ(I)> | 23.3 | 2 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 3.9 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | PEG8K 18%, CaAcetate 0.2M, NaCacod 0.1m, pH 6.5, 10mM L-Glutamic acid, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
