3ISU
Crystal structure of the RGC domain of IQGAP3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97711 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 58.120, 58.120, 78.292 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 1.880 |
| R-factor | 0.209 |
| Rwork | 0.207 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3iez |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.612 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 1.910 |
| High resolution limit [Å] | 1.880 | 5.100 | 1.880 |
| Rmerge | 0.086 | 0.042 | 0.942 |
| Number of reflections | 12943 | ||
| <I/σ(I)> | 10.3 | ||
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 20.5 | 18.9 | 18.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 25% PEG3350, 0.2M lithium sulfate, 0.1 Bis-Tris, pH 6.5, vapor diffusion, sitting drop, temperature 291K |
