3ISR
The Crystal Structure of a Putative Cysteine Protease from Cytophaga hutchinsonii to 1.9A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-08-07 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9794 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 90.716, 115.240, 131.262 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.280 - 1.900 |
R-factor | 0.189 |
Rwork | 0.187 |
R-free | 0.22000 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.207 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | REFMAC (refmac_5.5.0102) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
Rmerge | 0.073 | 0.049 | 0.689 |
Number of reflections | 108506 | ||
<I/σ(I)> | 8.7 | ||
Completeness [%] | 99.5 | 97.2 | 100 |
Redundancy | 4.9 | 4.8 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.9 | 289 | 20% PEG 3350, 0.2M Sodium thiocyanate, pH 6.9, vapor diffusion, temperature 289K |