3ISP

Crystal structure of ArgP from Mycobacterium tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9798
Spacegroup name	I 21 21 21
Unit cell lengths	85.800, 116.740, 136.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.606 - 2.700
R-factor	0.212
Rwork	0.207
R-free	0.27100
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.110
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.063	0.602
<I/σ(I)>	25.8	2.6
Completeness [%]	98.0	93

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	290	1.6M Na2HPO4/NaH2PO4, pH5.8, VAPOR DIFFUSION, HANGING DROP, temperature 290K