3ISL
Crystal structure of ureidoglycine-glyoxylate aminotransferase (pucG) from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-15 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.2 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 102.400, 95.990, 101.290 |
| Unit cell angles | 90.00, 114.23, 90.00 |
Refinement procedure
| Resolution | 27.690 - 2.060 |
| R-factor | 0.1961 |
| Rwork | 0.193 |
| R-free | 0.24689 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jvo |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.052 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.690 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.056 | 0.409 |
| Number of reflections | 54886 | |
| <I/σ(I)> | 9.6 | 2.5 |
| Completeness [%] | 97.9 | 85.8 |
| Redundancy | 3.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 9 | 293 | 30% PEG MME550, 0.1M NaCl, 0.1M Bicine, pH9.0, VAPOR DIFFUSION, temperature 293K |
