3IS1
Crystal structure of functional region of UafA from Staphylococcus saprophyticus in C2 form at 2.45 angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32B2 |
Synchrotron site | SPring-8 |
Beamline | BL32B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-11-03 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 1.00000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 99.585, 110.442, 49.547 |
Unit cell angles | 90.00, 106.49, 90.00 |
Refinement procedure
Resolution | 19.830 - 2.450 |
R-factor | 0.20905 |
Rwork | 0.206 |
R-free | 0.24574 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.868 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | DM |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.540 |
High resolution limit [Å] | 2.450 | 2.450 |
Number of reflections | 18642 | |
Completeness [%] | 98.7 | 90.4 |
Redundancy | 4.7 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 300mM tri-lithium citrate, 20% PEG 3350, VAPOR DIFFUSION, temperature 293K |