3IRT
Crystal Structure of the I93M Mutant of Ubiquitin Carboxy-terminal Hydrolase L1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-06 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0331 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 109.938, 109.938, 79.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.748 - 2.799 |
| R-factor | 0.2126 |
| Rwork | 0.211 |
| R-free | 0.25380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | One subunit of the asymmetric unit of 2ETL |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.641 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.750 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.111 | 0.540 |
| Number of reflections | 11988 | |
| <I/σ(I)> | 19.98 | 1.78 |
| Completeness [%] | 96.1 | 70 |
| Redundancy | 12.8 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 2.4 M Ammonium Sulfate, 0.1 M TRIS Hydrochloride, 0.1 M Sodium Sodium Malonate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
