3IRT
Crystal Structure of the I93M Mutant of Ubiquitin Carboxy-terminal Hydrolase L1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-06 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0331 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 109.938, 109.938, 79.040 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.748 - 2.799 |
R-factor | 0.2126 |
Rwork | 0.211 |
R-free | 0.25380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | One subunit of the asymmetric unit of 2ETL |
RMSD bond length | 0.003 |
RMSD bond angle | 0.641 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.750 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.111 | 0.540 |
Number of reflections | 11988 | |
<I/σ(I)> | 19.98 | 1.78 |
Completeness [%] | 96.1 | 70 |
Redundancy | 12.8 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 2.4 M Ammonium Sulfate, 0.1 M TRIS Hydrochloride, 0.1 M Sodium Sodium Malonate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |