3IRP
Crystal structure of functional region of UafA from Staphylococcus saprophyticus at 1.50 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-29 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.935, 126.926, 45.830 |
| Unit cell angles | 90.00, 96.75, 90.00 |
Refinement procedure
| Resolution | 19.180 - 1.500 |
| R-factor | 0.16745 |
| Rwork | 0.165 |
| R-free | 0.18602 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3is1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.186 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 68192 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 30% PEG MME2000, 70mM potassium thiocyanate, VAPOR DIFFUSION, temperature 293K |
