3IR9
C-terminal domain of Peptide Chain Release Factor from Methanosarcina mazei.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | H 3 |
| Unit cell lengths | 116.124, 116.124, 71.454 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.100 - 2.207 |
| R-factor | 0.1583 |
| Rwork | 0.156 |
| R-free | 0.20340 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.896 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.100 | 2.250 |
| High resolution limit [Å] | 2.207 | 2.210 |
| Rmerge | 0.076 | 0.793 |
| Number of reflections | 35874 | |
| <I/σ(I)> | 8.7 | 2.02 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.7 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.1 M HEPES buffer, 70% MPD, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
