3IQL
Crystal structure of the rat endophilin-A1 SH3 domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-02 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97720 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 64.338, 34.156, 62.729 |
Unit cell angles | 90.00, 107.79, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.400 |
R-factor | 0.12837 |
Rwork | 0.127 |
R-free | 0.15358 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gcq chain A Non-conserved residue pruned to CB atom using CHAINSAW |
RMSD bond length | 0.012 |
RMSD bond angle | 1.402 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.500 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.031 | 0.071 |
Number of reflections | 23667 | |
<I/σ(I)> | 30.2 | 11.1 |
Completeness [%] | 96.3 | 85.5 |
Redundancy | 3 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277 | 20% methyl-2,4-pentanediol, 0.1M sodium acetate, 0.05M calcium chloride, 10mM 2-mercaptoethanol, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |