3IQI
Structure of O-Acetylserine Sulfhydrylase in Complex with Peptide MNENI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 130 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-01-16 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 41 |
| Unit cell lengths | 112.264, 112.264, 45.835 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.080 - 1.700 |
| R-factor | 0.1737 |
| Rwork | 0.172 |
| R-free | 0.20439 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y7l |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.282 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.080 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.033 | 0.152 |
| Number of reflections | 29196 | |
| <I/σ(I)> | 28.1 | 6.2 |
| Completeness [%] | 97.3 | 85.9 |
| Redundancy | 3.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | HEPES, Ammonium sulfate, PEG 400, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
