3IQG
Structure of O-Acetylserine Sulfhydrylase in Complex with Peptide MNWNI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 130 |
Detector technology | IMAGE PLATE |
Collection date | 2009-05-29 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | I 41 |
Unit cell lengths | 112.474, 112.474, 45.928 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.140 - 1.900 |
R-factor | 0.16823 |
Rwork | 0.166 |
R-free | 0.20534 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1y7l |
RMSD bond length | 0.015 |
RMSD bond angle | 1.409 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | REFMAC |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.040 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.029 | 0.103 |
Number of reflections | 22692 | |
<I/σ(I)> | 31.8 | 8.6 |
Completeness [%] | 99.2 | 95.7 |
Redundancy | 3.8 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 10.5 | 298 | CAPS, Ammonium Sulfate, Lithium Sulfate, pH 10.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |